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iem fixative  (Servicebio Inc)

 
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    Servicebio Inc iem fixative
    Iem Fixative, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iem+fixative/fixative+iem/pmc12808913-65-20-25
    Average 86 stars, based on 1 article reviews
    iem fixative - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Saline:

    Article Title: Serine protease Rv2569c facilitates transmission of Mycobacterium tuberculosis via disrupting the epithelial barrier by cleaving E-cadherin
    Article Snippet: .. Following resuspension and washing with phosphate-buffered saline (PBS), the cellular pellet was fixed with IEM fixative (Servicebio, Wuhan, China) for 2 h at room temperature while being shielded from light. ..

    other:

    Article Title: Bacterial colonization contributes to pathological scar formation via the regulation of inflammatory response.
    Article Snippet: The slides were visualized using a NikonDS-U3 automated slide scanner (DA-U3, Nikon, Shanghai, China).

    Injection:

    Article Title: In vivo imaging reveals exosome-mediated intercellular communication in lens development.
    Article Snippet: Finally, the sections were examined and imaged via a TEM (JEM-1400Flash, Rigaku, Japan). .. Zebrafish embryos injected with the cryaa-Cd63-AcGFP plasmid at 3.5 dpf were fixed in IEM fixative (G1124, Servicebio). ..

    Plasmid Preparation:

    Article Title: In vivo imaging reveals exosome-mediated intercellular communication in lens development.
    Article Snippet: Finally, the sections were examined and imaged via a TEM (JEM-1400Flash, Rigaku, Japan). .. Zebrafish embryos injected with the cryaa-Cd63-AcGFP plasmid at 3.5 dpf were fixed in IEM fixative (G1124, Servicebio). ..

    Centrifugation:

    Article Title: Extracellular DNase MAP3916c attacks the neutrophil extracellular traps and is needed for Mycobacterium avium subsp. paratuberculosis virulence.
    Article Snippet: Extracellular DNases/nucleases are important virulence factors in many bacteria.. However, no DNase/nucleases have been reported in Mycobacterium avium subsp. paratuberculosis (MAP), which is a pathogen of paratuberculosis.. Genome analyses of MAP K-10 revealed that the map3916c gene putatively encodes a nuclease.



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    specialized fixative for immunoelectron microscopy (iem)  (Servicebio Inc)
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    Servicebio Inc specialized fixative for immunoelectron microscopy (iem)
    <t>Immunoelectron</t> micrograph of beta-conglycinin hydrolysates absorbed into cell monolayers. The IPEC-J2 cells were cultured in a 6-well plate using DMEM/F12 complete medium for 6 days to establish dense cell monolayers. Following this, the cells were incubated with a DMEM medium containing 1.0 mg/mL of beta-conglycinin hydrolysates for 8 h. After incubation, the cell monolayers were thoroughly washed, fixed, and then processed according to the standard procedure for immunoelectron <t>microscopy.</t> The resulting ultrathin sections were photographed using a transmission electron microscope. The immunogold-labeled beta-conglycinin fragments are presented as black dots in the micrograph.
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    Immunoelectron micrograph of beta-conglycinin hydrolysates absorbed into cell monolayers. The IPEC-J2 cells were cultured in a 6-well plate using DMEM/F12 complete medium for 6 days to establish dense cell monolayers. Following this, the cells were incubated with a DMEM medium containing 1.0 mg/mL of beta-conglycinin hydrolysates for 8 h. After incubation, the cell monolayers were thoroughly washed, fixed, and then processed according to the standard procedure for immunoelectron microscopy. The resulting ultrathin sections were photographed using a transmission electron microscope. The immunogold-labeled beta-conglycinin fragments are presented as black dots in the micrograph.

    Journal: International Journal of Molecular Sciences

    Article Title: A Quantity-Dependent Nonlinear Model of Sodium Cromoglycate Suppression on Beta-Conglycinin Transport

    doi: 10.3390/ijms25126636

    Figure Lengend Snippet: Immunoelectron micrograph of beta-conglycinin hydrolysates absorbed into cell monolayers. The IPEC-J2 cells were cultured in a 6-well plate using DMEM/F12 complete medium for 6 days to establish dense cell monolayers. Following this, the cells were incubated with a DMEM medium containing 1.0 mg/mL of beta-conglycinin hydrolysates for 8 h. After incubation, the cell monolayers were thoroughly washed, fixed, and then processed according to the standard procedure for immunoelectron microscopy. The resulting ultrathin sections were photographed using a transmission electron microscope. The immunogold-labeled beta-conglycinin fragments are presented as black dots in the micrograph.

    Article Snippet: Following centrifugation at 1000 rpm for 2 min, a specialized fixative for immunoelectron microscopy (IEM) (Servicebio, Wuhan, China) was added to the cell pellet.

    Techniques: Cell Culture, Incubation, Immuno-Electron Microscopy, Transmission Assay, Microscopy, Labeling