Journal: International Journal of Molecular Sciences
Article Title: A Quantity-Dependent Nonlinear Model of Sodium Cromoglycate Suppression on Beta-Conglycinin Transport
doi: 10.3390/ijms25126636
Figure Lengend Snippet: Immunoelectron micrograph of beta-conglycinin hydrolysates absorbed into cell monolayers. The IPEC-J2 cells were cultured in a 6-well plate using DMEM/F12 complete medium for 6 days to establish dense cell monolayers. Following this, the cells were incubated with a DMEM medium containing 1.0 mg/mL of beta-conglycinin hydrolysates for 8 h. After incubation, the cell monolayers were thoroughly washed, fixed, and then processed according to the standard procedure for immunoelectron microscopy. The resulting ultrathin sections were photographed using a transmission electron microscope. The immunogold-labeled beta-conglycinin fragments are presented as black dots in the micrograph.
Article Snippet: Following centrifugation at 1000 rpm for 2 min, a specialized fixative for immunoelectron microscopy (IEM) (Servicebio, Wuhan, China) was added to the cell pellet.
Techniques: Cell Culture, Incubation, Immuno-Electron Microscopy, Transmission Assay, Microscopy, Labeling